E.coli RNA entrapped in large, unilamellar liposomes is partially exposed

R SIRDESHMUKH, D VIJAYARANGAM & P M BHARGAVA

Centre for Cellular and Molecular Biology Hyderabad, 500007, A.P., India

Published in: Indian Journal of Biochemistry & Biophysics, 1984, 21, 293-298.

Entrapment of 3H-labelled total E.coli RNA in unilamellar liposomes prepared by the ether infusion method, and the spatial status of the entrapped RNA, have been studied. Separation of the liposomes from the unentrapped RNA was achieved by direct chromatography of the RNA-containing liposomes on Biogel A-50. The entrapped RNA contained all the major species (23S, 16S and 4S) and amounted to 20-30% of the input RNA. Ten to forty per cent of the RNA in the liposomes purified on the Biogel column could be degraded on treatment of the liposomes with RNase A. The rest (60-90%) of the entrapped RNA was resistant to further treatment with the nuclease and consisted only of fragmented RNA, largely 4S, in most of the experiments. It appears that the RNA entrapped in liposomes prepared by the ether infusion method, spans the lipid bilayer so that a part of the molecule sticks out of the bilayer and can be degraded by RNase A.

PMID:6085832

Keywords:
Adsorption, Escherichia coli/analysis, Liposomes/analysis, RNA, Bacterial/analysis

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E.coli RNA entrapped in large, unilamellar liposomes is partially exposed. R SIRDESHMUKH, D VIJAYARANGAM & P M BHARGAVA. Indian Journal of Biochemistry & Biophysics. 21, 293-298.

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