The comparative utilization of uracil-2-C14 by liver intestinal mucosa and Flexner-Joebling carcinoma in the rat

C HEIDELBERGER, K C LEIBMAN, E HARBERS & P M BHARGAVA

Published in: Cancer Research, 1957,17, 399-403.

Although it has been shown that the injection of N16-labeled pyrimidines into normal adult rats does not lead to appreciable labeling of the total nucleic acids (1, 19), Rutman et al. (20) have made the interesting observation that uracil-2-C14 is incorporated into the nucleic acids of rat hepatomas induced by 2-acetylaminofluorene to a considerably greater extent than into those of normal liver. Because of the possible chemotherapeutic implications of this finding (10) and as a continuation of the work in this laboratory on the biosynthesis of nucleic acids (11, 16, 18, 28), experiments were undertaken to determine whether uracil is also incorporated into the nucleic acids of transplantable tumors or into other normal growing tissues. The three tissues chosen were rat liver (both of normal and tumor-bearing animals), intestinal mucosa, and the Flexner-Tobling carcinoma. The results show that the transplanted tumor exhibits an elevated utilization of uracil for nucleic acid biosynthesis comparable to that found by Rutman et al. (20) for the primary hepatoma and that uracil is also well utilized by intestinal mucosa. An assessment of the comparative importance of uracil and orotic acid as building blocks in these tissues has been made, and some in vitro experiments designed to give information of the mechanisms of these conversions have been carried out.

PMID: 13437303

Keywords:
Intestines/metabolism, Liver/metabolism, Neoplasms/metabolism, Uracil/metabolism

HOW TO CITE

The comparative utilization of uracil-2-C14 by liver intestinal mucosa and Flexner-Joebling carcinoma in the rat. C HEIDELBERGER, K C LEIBMAN, E HARBERS & P M BHARGAVA. Cancer Research, 1957,17, 399-403.

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