Effect of acid precipitation on the solubility and the electrophoretic characteristics of plasma proteins

K A ABRAHAM & P M BHARGAVA

Published in: Indian Journal of Chemistry, 1963, 1, 124-130.

Abstract:

The effect of precipitation by several acids on the solubility and the electrophoretic characteristics of rabbit plasma protein has been studied. The amount of water-soluble or of salt-soluble proteins in the plasma is not altered on precipitation with 5 per cent perchloric, hydrochloric or nitric acids followed, in the last two cases, by further treatment with trichloroacetic acid to precipitate the mineral acid-soluble proteins. Trichloroacetic acid( 5 per cent) renders the salt-soluble proteins of plasma (the globulins) water-soluble, without altering their electrophoretic mobility; treatment of the tricholoroacetic acid precipate with 5 percent sodium chloride renders a part of the albumin precipitable at half saturation with ammonium sulphate.

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Effect of acid precipitation on the solubility and the electrophoretic characteristics of plasma proteins. K A ABRAHAM & P M BHARGAVA. Indian Journal of Chemistry, 1963, 1, 124-130.

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