The effect of acids on proteins.

K A ABRAHAM & P M BHARGAVA

Published in: Proceedings of the Symposium on Proteins held at Mysore, August 14-16, 1961 (CFTRI, Mysore) 1961, 37-46.

Abstract:

This investigation had its origin in an accidental finding that seminal plasma proteins; after precipitation with 5 per cent trichloroaceticacid (TCA) and washing twice with sodium chloride, could be dissolved, almost completely, in, distilled water. Since denaturation of proteins is usually understood to result in a loss of solubility it appeared that a major portion of the plasma proteins was not denatured by 5 percent TCA, although the proteins were precipitated. .Our observations on the effect of TCA on seminal plasma proteins are recorded in this paper.

Since seminal plasma is analogous to blood plasma we have also studied the action of various acids on blood plasma proteins.

It has been recently shown that the architecture of a protein molecule as it occurs in the native state, is not essential for its enzymic activity. Thus chymotrypsin1, trypsin2, papain3 , and ribonuclease4 have been shown to retain l their enzymic activity even after considerable degradation and consequent reduction in molecular weight. The antigenic properties of bovine serum albumin (BSA) are unaltered even after removal of a large peptide by the action of trypsin5. Ovalbumin spread in the forn of 9 A0 thick monolayers under which condition the molecule should be drastically modified, has been shown to retain the ability to combine with its specific antibody6.

HOW TO CITE

The effect of acids on proteins. K A ABRAHAM & P M BHARGAVA. Proceedings of the Symposium on Proteins held at Mysore, August 14-16, 1961 (CFTRI, Mysore) 1961, 37-46.

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