The effect of ribonuclease on rat-liver parenchymal cells in suspension

B VIJAYA KUMAR & P M BHARGAVA

Published in: Biochemie, 1973, 55, 661-670.

Abstract:

  1. Rat-liver parenchymal cells in suspension have been shown to take up 131I -ribonuclease.
  2. The nuclease taken up degrades up to 40 p. cent of the cellular RNA to acid-soluble material; this RNA appears different from the RNA degraded by the endogenous nuclease activated on dispersion of liver tissue to a single cell suspension and incubation at 37°C.
  3. The ribonuclease taken up at 28°C, partially depolymerises cellular rRNA in such a way that the overall structural integrity or the biological activity of the ribosomes not affected; cellular tRNA activity also is not affected.

PMID: 4797820

Keywords:
Centrifugation, Density Gradient, Iodine Radioisotopes, Kinetics, Liver/cytology, Liver/metabolism, Phosphorus Radioisotopes, Poly U/pharmacology, RNA/analysis, RNA/metabolism, RNA, Ribosomal/metabolism, RNA, Transfer/metabolism, Ribonucleases/metabolism, Ribosomes/analysis, Structure-Activity Relationship, Sucrose, Temperature

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The effect of ribonuclease on rat-liver parenchymal cells in suspension. B VIJAYA KUMAR & P M BHARGAVA. Biochemie, 1973, 55, 661-670.

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