Effect of spermine, EDTA, urea and high salt on the activity of ribonuclease SPL on Mg2+-containing undenatured total E.coli and rat liver RNAs

E S P REDDY, D VIJAYARANGAM & P M BHARGAVA

Centre for Cellular and Molecular Biology, Hyderabad, India

Published in: Japanese Journal of Biochemistry, 1983, 93, 207-210.

Abstract:

Using RNAase SPL, a recently described nuclease from bovine seminal plasma, that can recognize certain Mg2+ -dependent structural features in native naturally occurring RNAs, it is shown that these features are wholly or partially destroyed by 8 M urea, 1 mM spermine, 0.35 M KCl or NaCl, and 10 mM EDTA, urea and spermine being the most effective and EDT A the least; these features are not destroyed by heating and rapid cooling unless a medium with a high ionic strength is used. Since the buffer used so far for reconstitution of ribosomes contains 0.35 M KCl, it seems possible that a partial opening up of ribosomal RNAs may be necessary for ribosome assembly.

PMID: 6188753

Keywords:

Edetic Acid/pharmacology, Endoribonucleases/metabolism, Escherichia coli/metabolism, Liver/metabolism, Magnesium/isolation & purification, Nucleic Acid Denaturation, Osmolar Concentration, RNA/metabolism, RNA, Bacterial/metabolism, Rats, Spermine/pharmacology, Urea/pharmacology

HOW TO CITE

Effect of spermine, EDTA, urea and high salt on the activity of ribonuclease SPL on Mg2+-containing undenatured total E.coli and rat liver RNAs. E S P REDDY, DVIJAYARANGAM & P M BHARGAVA. Japanese Journal of Biochemistry, 1983, 93, 207-210.

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