Evidence for the presence of a cell-surface ribonuclease in mechanically prepared rat-liver cells in suspension

R SIRDESHMUKH & P M BHARGAVA

Centre for Cellular and Molecular Biology Hyderabad, 500007, A.P., India

Published in: Bioscience Reports, 1982, 2, 751-760.

Abstract:

Rat-liver parenchymal cells obtained in suspension by a mechanical method are shown to contain a cell-surface nuclease(s) that rapidly degrades exogenously added total Escherichia coli RNA. However, no add-soluble products are formed; all the degradation products in the incubation medium sediment in the 4-5S RNA region on a sucrose density gradient. A part of the degraded RNA seems to be taken up by the cells; the uptake of the degradation products, presumably derived from rRNAs, is more than that of purified 4-5S RNA. Most of the RNA taken up by the cell sediments in the 4-5S region; only a small proportion is degraded to acid-soluble material within the cell.

PMID: 7171742

Keywords:
Cell Membrane/enzymology, Centrifugation, Density Gradient, Kinetics, Liver/enzymology, Molecular Weight, Rats, Inbred Strains, Ribonucleases/isolation & purification, Ribonucleases/metabolism

HOW TO CITE

Evidence for the presence of a cell-surface ribonuclease in mechanically prepared rat-liver cells in suspension. R SIRDESHMUKH & P M BHARGAVA. Bioscience Reports, 1982, 2, 751-760.

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