Immunological identification of seminalplasmin in tissue extracts of sex glands of bull

S SHIVAJI1, P M BHARGAVA1 & K H SCHEIT2

1Centre for Cellular and Molecular Biology, Hyderabad, India

2Max-Planck-Institut fuer Biophysikalische Chemie, Abteilung Molekulare Biologie Am Fassberg, 3400 Goettingen, West Germany

Published in: Biology of Reproduction, 1984, 30, 1237-1242.

Abstract:

Using immunoglobulin G (IgG) antibodies raised against highly purified, homogeneous seminalplasmin, an antimicrobial protein of bovine seminal plasma, it has been shown that bovine ampullae, gland vesicularis and corpus prostate, but not testes and epididymis, contain seminalplasmin. The content as estimated by radioimmunoassay employing 125I-seminalplasmin was: ampullae, 267 +/- 13; gland vesicularis, 275 +/- 14; and corpus prostate, 445 +/- 22 micrograms per g wet weight of the tissue. Seminalplasmin, as characterized by high-performance liquid chromatography and in vivo inhibition of RNA synthesis in E. coli, was isolated from gland vesicularis. The seminalplasmin content of bovine seminal plasma was shown to be 1%. A chymotryptic peptide of seminalplasmin comprising residues 1-13 from the amino terminus was found to compete with 125I-seminalplasmin for binding to anti-seminalplasmin IgG.

PMID: 6733212 | DOI: 10.1095/biolreprod30.5.1237

Keywords:
Antibodies/immunology, Cattle, Epididymis/analysis, Genitalia, Male/analysis, Male, Prostate/analysis
Proteins/analysis, Proteins/immunology, Radioimmunoassay, Seminal Vesicle Secretory Proteins, Seminal Vesicles/analysis, Testis/analysis, Vas Deferens/analysis

HOW TO CITE

Immunological identification of seminalplasmin in tissue extracts of sex glands of bull. S SHIVAJI, P M BHARGAVA & K H SCHEIT. Biology of Reproduction, 1984, 30, 1237-1242.

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