Incorporation of C14-aminoacids into the proteins of bull spermatozoa

P M BHARGAVA, M W H BISHOP & T S WORK

National Institute for Medical Research, Mill Hill, London, N.W. 7

Published in: Biochemical Journal, 1959, 73, 247-256.

Abstract:

During the last two decades, a considerable amount of evidence has accumulated to suggest that ribonucleic acid plays an essential role in protein biosynthesis (see Brachet, 1955). Since bull spermatozoa are virtually devoid of ribonucleic acid (Bhargava, Bishop & Work, 1959), they would be expected to be incapable of protein synthesis. It has already been shown, however, that washed bull spermatozoa can incorporate radioactive amino acids into their proteins (Bhargava, 1957); these observations are extended and evaluated in the present paper. We have also studied amino acid incorporation by unwashed spermatozoa in seminal plasma since this system more closely resembles that met with in vivo. Marked differences were observed between the washed and the unwashed cells in both the rates and the patterns of incorporation of amino acid into protein. The effect of several metabolic inhibitors on incorporation by both washed and unwashed cells was also investigated. For comparative purposes, the rate of in­corporation of amino acids into proteins of reticulocytes has been measured under similar conditions to those used for washed spermatozoa.

PMID: 13849051

Keywords:
Amino Acids/metabolism, Proteins/metabolism, Spermatozoa/metabolism

HOW TO CITE

Incorporation  of C14-aminoacids into the proteins of bull spermatozoa.  P M BHARGAVA, M W H BISHOP & T S WORK.  Biochemical Journal, 1959, 73, 247-256.

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