Incorporation of radioactive phosphorus into the ribonucleic acid subfractions derived from guinea-pig liver microsomes

P M BHARGAVA, J L SIMKIN & T S WORK

National Institute for Medical Research, Mill Hill, London, N.W. 7

Published in: Biochemical Journal, 1958, 68, 265-269.

Abstract:

It is now generally accepted that there is a particularly rapid protein turnover in the microsome fraction of liver in various animals. We have already shown (Simkin & Work, 1957a) that the microsome fraction of guinea-pig liver can be separated by successive extractions with solutions of varying ionic strength and pH into several distinct subfractions, each containing proteins with different turnover rates. Two of these subfractions, B and C, appeared to be ribonucleoproteins, and after injection of [14C] amino acids into normal guinea pigs the protein components of one of these two fractions (C) became labelled more rapidly than the other. Since a considerable amount of evidence has accumulated recently which suggests that ribonucleic acid (RNA) plays a special role in protein synthesis (Brachet, 1955), it seemed desirable to compare RNA turnover with protein turnover in the various subfractions from liver microsomes.

In the experiments described here, radioactive orthophosphate was injected into guinea pigs which were killed at appropriate time intervals. The microsome fraction was isolated and subfractionated as described earlier and the rate of incorporation of 32P into the RNA of the different microsome subfractions was measured. It was found that in sub-fraction C, which had the highest rate of protein turnover, the rate of incorporation of 32P into RNA was below that observed for any other microsomal subfraction.

PMID: 13522612

Keywords:
Liver/metabolism
Phosphorus/metabolism
RNA/metabolism

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Incorporation of radioactive phosphorus into the ribonucleic acid subfractions derived from guinea-pig liver microsomes. P M BHARGAVA, J L SIMKIN & T S WORK, Biochemical Journal, 1958, 68, 265-269.

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