Isolation and characterization of autolysis-defective mutants of Escherichia Coli that are resistant to the lytic activity of seminalplasmin

S N CHITNIS, K S N PRASAD & P M BHARGAVA

Centre for Cellular and Molecular Biology, Hyderabad, India.

Published in: Journal of General Microbiology, 1990, 136, 463-469.

Abstract:

Two temperature-sensitive autolysis-defective mutants of Escherichia coli were isolated and shown to be resistant to lysis induced by seminalplasmin, an antimicrobial protein from bovine seminal plasma, as well as to lysis induced by ampicillin, D-cycloserine and nocardicin, at 37 or 42 degrees C but not at 30 degrees C. The mutants were, however, sensitive to inhibition of RNA synthesis by seminalplasmin even at the nonpermissive temperature. Temperature-resistant revertants of the mutants were sensitive to lysis induced by the various antibiotics at 37 or 42 degrees C. The mutations in both strains were mapped at 58 min on the E. coli linkage map. The lysis resistance of the mutants was phenotypically suppressed by the addition of NaCl. Partial suppression of the lysis-resistant phenotype was also observed in a relA genetic background.

PMID: 1697323

HOW TO CITE

Isolation and characterization of autolysis-defective mutants of Escherichia Coli that are resistant to the lytic activity of seminalplasmin. S N CHITNIS, K S N PRASAD & P M BHARGAVA. Journal of General Microbiology, 1990, 136, 463-469.

READ THIS ARTICLE ONLINE OR ↓ DOWNLOAD A COPY

Download the full Paper from PMB memoir

↪ Full Text Online Link 1
➽ Full Text Online Link 2 ↪ Download PDF

Having Trouble Downloading? ↪ Watch here how to download this article  ❙❙ ❚❚ ►

Leave a Reply

Your email address will not be published. Required fields are marked *