Isolation and characterization of newly synthesized RNA and protein in mature bovine spermatozoa and the effect of inhibitors on these syntheses

E PREMKUMAR & P M BHARGAVA

Published in: Indian Journal of Biochemistry & Biophysics, 1974, 10, 239-253.

Abstract:

Freshly ejaculated mature bovinespermatozoa, .freed of seminal plasma, have been shown to synthesize RNAs which resemble bacterial rRNAs and tRNA, and a 16 S RNA which is not rRNA (16 S non-rRNA). From the behaviour of the newly synthesized RNA on four commonly used fractionation systems, its hybridization with somatic cell, and mitochondrial DNAs and the effect of drugs on its synthesis, it has been concluded that the RNAs synthesized by spermatozoa are transcription products of their mitochondrial DNA. The 16S non-rRNA is found to be the most susceptible to degradation. The spermatozoa have also been shown to synthesize only a small number of proteins, probably only 5, all on a mitochondrial RNA template. The results show that in bovine spermatozoa, transcription and translation are largely, if not exclusively, confined to their mitochondrial DNA.

PMID:4792940

Keywords:
Anti-Bacterial Agents/pharmacology, DNA, Mitochondrial/metabolism, Depression, Chemical, Nucleic Acid Hybridization, Phosphorus Radioisotopes, Protein Biosynthesis, Proteins/isolation & purification, RNA/biosynthesis, RNA/isolation & purification, Spermatozoa/metabolism, Transcription, Genetic

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Isolation and characterization of newly synthesized RNA and protein in mature bovine spermatozoa and the effect of inhibitors on these syntheses. E PREMKUMAR & P M BHARGAVA. Indian Journal of Biochemistry & Biophysics, 1974, 10, 239-253.

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