Preparation of kidney cell suspensions and respiration of kidney and liver cell suspensions

B THIMMAP-PAYYA, R R REDDY & P M BHARGAVA

Published in: Experimental Cell Research, 1970, 63, 303-310.

Abstract:

Preparation of kidney cell suspensions by two methods, one mechanical which yielded single cells only, and the other enzymatic which yielded a mixture of single cells and fragmented tubules, is described. The recovery of tissue dry weight in the single cells was more by the former than by the latter method, but did not exceed 5 %. The QO2 of mechanically dispersed cells and of enzymatically dispersed cells and tubules was 4.8, 5.7 and 15.9 respectively, in comparison with 5.0 and 5.3 for unperfused and perfused-kidney slices respectively; the respiration was linear in all the cases for at least 4 h. The respiration of mechanically dispersed kidney cells was not inhibited by calcium and was independent of cell concentration; in respect of the former property, these cells resembled enzymatically dispersed liver cells, and in that of the latter, mechanically dispersed liver cells.

PMID: 4321428

Keywords:
Culture Media, Culture Techniques, DNA/analysis, Hyaluronoglucosaminidase, Kidney/cytology, Kidney/metabolism, Liver/cytology, Liver/metabolism, Methods, Microbial Collagenase, Rabbits

HOW TO CITE

Preparation of kidney cell suspensions and respiration of kidney and liver cell suspensions. B THIMMAP-PAYYA, R R REDDY & P M BHARGAVA. Experimental Cell Research, 1970, 63, 303-310.

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