Preparation, properties and uses of single cell suspensions from normal tissues

| March 22, 2015 | 0 Comments

P M BHARGAVA

Published in: Science & Culture (Calcutta), 1968, 34 (Suppl..), 105-127.

Abstract:

There are five reasons for my choosing to present a review of current information on the above subject at this occasion. Firstly, cell suspensions represent an important level of organisation between the whole tissue and its cell-free homogenate. Secondly, as we shall see later, cell suspensions provide a unique tool for obtaining a variety of information in many areas of biology. Thirdly, I feel that the subject is very appropriate to the occasion, as in reviewing it; I would be led to discussion of certain aspects of relationship between structure and function. Fourthly, cell suspensions have interested .me and my colleagues in my laboratory now for over 8 years. Lastly, to my knowledge, such a review has not been presented till now either orally or in writing, excepting one by Rinaldini1on isolation of living cells from animal tissues published ten years ago, and a few other brief descriptions of the methods used for the preparation of single cell suspensions included in reviews on other subjects (inter alia)2.

This survey is only representative and not exhaustive. Studies on cells which normally exist as single cells in the animal (like blood cells, spermatozoa, ascites tumour cells, etc.) are excluded.

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Preparation,  properties  and uses of single cell suspensions from normal tissues.  P M BHARGAVA.  Science & Culture (Calcutta), 1968, 34 (Suppl..), 105-127.

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