Protein and nucleic acid metabolism of spermatozoa

| March 22, 2015 | 0 Comments

P M BHARGAVA

Published in: Proceedings of the Fifth Inter-national Congress on Animal Reproduction and Artificial insemination held at Trento, Italy, 1964), 1965, 7, 362-364.

Abstract:

Several years ago we had observed that bull spermatozoa, which have no chemically detectable RNA (1, 2) can, incorporate labelled amino acids into their proteins at a rate comparable to that obtained with tissue slices (3-5). Since then, work on this problem in our laboratory has been directed towards the following:

1) to determine whether RNA is present in mammalian spermatozoa in trace amounts undetectable by the usual chemical methods;

2) to determine if the incorporation of radioactive amino acids into proteins of the spermatozoa represents true intracellular protein synthesis, and if so, to find the reason for the discrepancy observed by MARTIN and BRACHET (6) between the biochemical and auto-radiographic studies on incorporation of labelled amino acids in these cells (these authors could not detect any intracellular label in spermatozoa incubated with labelled amino acids and then autoradiographed);

3) to determine the nature of RNA, if any, in the spermatozoa;

4) to determine the nature of protein synthesised, if any, by the spermatozoa;

5) to elucidate the relationship between the RNA and protein syntheses, if any, by the spermatozoa;

6) to elucidate the biological function of the RNA and protein which may be synthesised by the spermatozoa.

The present status of our investigations and our future plans in respect to the above points are briefly reviewed below for comments and suggestions. It seems possible that our observations mentioned below on protein and nucleic acid syntheses by spermatozoa may have an implication in respect of control and regulation of fertility. Unless otherwise mentioned, the studies described are our own and have been carried out on bull, buffalo or/and goat spermatozoa.

HOW TO CITE

Protein and nucleic acid metabolism of spermatozoa. P M BHARGAVA. Proceedings of the Fifth Inter-national Congress on Animal Reproduction and Artificial insemination held at Trento, Italy, 1964), 1965, 7, 362-364.

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