A typical behaviour of ribonuclease SPL: different concentrations of the enzyme give different limit digests

E S P REDDY1, K H SCHEIT2 & P M BHARGAVA1

1Centre for Cellular and Molecular Biology, Hyderabad 500009, India
2Max-Planck Institut für Biophysikalische Chemie, Abteilung Molekulare Biologie, Am Fassberg, 3400 Göttingen-Nikolausberg FRG

Published in: FEBS Letters, 1981, 131, 377-380.

Abstract:

We have reported the isolation and characterisation of a new type of ribonuclease, RNase SPL, from bovine seminal plasma, which shows a RNase A-type activity on synthetic polyribonucleotides and on Mg2+ -containing heat-denatured or Mg2+ -depleted undenatured total Escherichia coli RNA, but which gives a limit digest containing only large (≥ Mr 10 000) fragments, with Mg2+-containing undenatured (native) total E. coli RNA [1]. Here we show that one cannot compensate for a lower concentration of RNase SPL by increasing the reaction time within the limits studied, even though the enzyme continues to be fully active during this period. RNase SPL appears to be the first enzyme described to exhibit this phenomenon.

PMID: 6170526

HOW TO CITE

A typical behaviour of ribonuclease SPL: different concentrations of the enzyme give different limit digests. E S P REDDY, K H SCHEIT & P M BHARGAVA. FEBS Letters, 1981, 131, 377-380.

READ THIS ARTICLE ONLINE OR ↓ DOWNLOAD A COPY

Download the full Paper from PMB memoir

↪ Link to Publisher
➽ Full Text Online ↪ Download PDF

Having Trouble Downloading? ↪ Watch here how to download this article  ❙❙ ❚❚ ►

Tags: ,

Leave a Reply

Your email address will not be published. Required fields are marked *