Uptake of exogenous RNA by rat-liver parenchymal cells in suspension prepared by the colleagenase method

R SIRDESHMUKH & P M BHARGAVA

Centre for Cellular and Molecular Biology Hyderabad, 500007, A.P., India

Published in: Indian Journal of Biochemistry & Biophysics, 1983, 20, 121-126.

Abstract:

Rat-liver parenchymal cells in suspension, prepared by the collagenase method have been found to take up homologous and E. coli total RNA. The uptake of the purified rRNA from E. coli was more than that of total RNA or purified 4-5S RNA from E. coli A part of the RNA taken up was degraded by the recipient cells to acid-soluble components; the remaining sedimented, on a sucrose density gradient in the 4-5S region. Under conditions that led to the above degradation of the RNA taken up the exogenous RNA remaining in the medium as well as the host cell RNA did not undergo degradation, suggesting the existence of a mechanism in the cell which allows it to distinguish between its own and exogenous RNA.

PMID:6200424

Keywords:
Biological Transport, Escherichia coli, In Vitro Techniques, Liver/cytology, Liver/metabolism, Microbial Collagenase, RNA/metabolism, RNA, Bacterial/metabolism, Rats, Inbred Strains

HOW TO CITE

Uptake of exogenous RNA by rat-liver parenchymal cells in suspension prepared by the colleagenase method. R SIRDESHMUKH & P M BHARGAVA. Indian Journal of Biochemistry & Biophysics, 1983, 20, 121-126.

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