Variation in the antibacterial activity of bovine seminal plasma

P M BHARGAVA, K JAMIL, N SHYAMALA RAO, B S N MURTHY & V RAMASWAMY

Centre for Cellular and Molecular Biology Hyderabad, 500007, A.P., India

Published in: Indian Veterinary Journal, 1986, 63, 642-649.

Summary:

Nine hundred and seventy eight samples of bovine seminal plasma derived from semen of 125 animals belonging to two species and eight breeds, collected over a period of 12 months were assayed for their ability to inhibit the growth of E.coli in a synthetic medium, at five concentrations representing 9 to 150-fold dilution of the seminal plasma. Over 16-fold variation in the antibacterial activity of seminal plasma was found between the animals and often in the same animal. On an average, the antibacterial activity of seminalplasmin decreased in the following order Hallikar, Tharparkar, Ongole, Deoni, Jersey x Local, Fresian, Jersey, Murrah. At concentrations at which no inhibition of the growth of E.coli was obtained bovine seminal plasma usually stimulated the growth of E.coli.

Abstract:

Bovine seminal plasma (BSP) has been recently shown te contain a highly antimicrobial protein, seminalplasmin (Reddy and Bhargava 1979; Scheit et al., 1979; Bhargava 1981; Rao et al., 1983; Reddy et al., 1983; Theil and Scheit 1983a, b). BSP, in addition, contains another protein, called antiseminalplasmin, which has also been purified to homogeneity. (Bhargava et al. 1981; Bhargava loc cit.: Rao and Bhargava 1985). Antiseminalplasmin acts as an antagonist of seminalplasmin in regard to the latter’s ability to inhibit the growth of E. coli. The net antibacterial activity of a given sample of seminal plasma, therefore, would depend, at least partially, on the absolute and the relative concentrations of seminalplasmin and antiseminalplasmin.

This paper reports the results of estimation of the antimicrobial activity against E. coli, of 978 samples of BSP obtained from 125 animals of eight different breeds belonging to two different bovine species. An attempt has also been made to determine if a correlation exists between the net antibacterial activity of BSP and the fertility of the semen sample from which the seminal plasma is derived.

Keywords:

Cattle, Semen, reproduction, Antimicrobials, Animal physiology, Escherichia

HOW TO CITE

Variation in the antibacterial activity of bovine seminal plasma. P M BHARGAVA, K JAMIL, N SHYAMALA RAO, B S N MURTHY & V RAMASWAMY. Indian Veterinary Journal, 1986, 63, 642-649.

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